个人简介

永利集团88304官网生命科学学院教授

教育经历

1984年获得中国科技大学生物系分子生物学专业学士学位；1992年获得爱尔兰国立大学科克学院微生物学系博士学位；

研究方向

多年来，本实验室的工作得到国际同行的认可，与欧洲的几个实验室建立并维持着卓有成效的合作关系，如法国巴斯德研究所、英国帝国理工学院、英国John Innes研究所、爱尔兰Carlow理工学院、爱尔兰国立大学Cork学院、西班牙国家生物技术中心、德国比勒菲尔德大学等。主要得到国家自然科学基金、国家科技部“863”、“973”项目基金、中法先进合作项目、国家教育部基金资助。主要工作包括：大肠杆菌及相关细菌中的基因调控网络，尤其是碳代谢和氮代谢的调控偶联；大肠杆菌及相关细菌中的基因调控机理；植物与微生物相互作用的分子生物学及功能基因组学研究；生物修复领域的研究（功能基因的分离）；在大肠杆菌及相关细菌中大量表达外源基因技术等。取得的主要成就有，发现原核基因表达调控中碳代谢及氮代谢之间的新的偶联作用及其分子机理；首次发现“下游激活序列”对固氮基因的调控作用；获得了新型高效抗草苷膦基因，在核酸水平上与已知基因没有同源性，在氨基酸水平上也只有37％左右的同源性，具有与受专利保护的EPSPS基因保守区不同的序列特征。目前世界上70％的转基因植物含有抗除草剂基因，其中60％是草甘膦耐受型EPSPS基因,具有很大的应用前景。

代表性成果

1. Xiang, N., Guo, C., Liu, J., Xu, H., Dixon, R.,* Yang, J.,* and Wang, Y.P.* (2020) Using synthetic biology to overcome barriers to stable expression of nitrogenase in eukaryotic organelles. Proc Natl Acad Sci USA. 117 (28): 16537-16545. doi:10.1073/pnas.2002307117
2.Jiang, F., Li, N., Wang, X., Cheng, J., Huang, Y., Yang, Y., Yang, J., Cai, B., Wang, Y.P., Jin, Q., and Gao, N. (2019) Cryo-EM structure and assembly of an extracellular contractile injection system. Cell 177, 1–14. doi: 10.1016/j.cell.2019.02.020.
3. Yang, J., Xie, X., Xiang, N., Dixon, R.,* and Wang, Y.P.* (2018) A polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology. Proc Natl Acad Sci U S A. 201804992; DOI: 10.1073/pnas.1804992115 [Highlighted by Stefan Burén, Gema Ló pez-Torrejón, and Luis M. Rubio (2018) Extreme bioengineering tomeet the nitrogen challenge Proc Natl Acad Sci U S A. doi/10.1073/pnas.1812247115]
4. Zhu, M., Dai, X., Guo, W., Ge, Z., Yang, M., Wang, H., and Wang, Y.P.* (2017) Manipulating bacterial cell cycle and cell size by titrating the expression of ribonucleotide reductase. mBio 8: e01741-17..
5. Yang, J., Xie, X. Yang, M. Dixon, R.* and Wang, Y.P.* (2017) Modular electron transport chains from eukaryotic organelles function to support nitrogenase activity. Proc Natl Acad Sci U S A. 114: 3009-3011. doi:10.1073/pnas.1620058114 [Highlighted by Vicente, E.J. and Dean, D.R. Keeping the nitrogen-fixation dream alive. Proc Natl Acad Sci U S A. 2017, 114 (12): 3009-3011, doi:10.1073/pnas.1701560114. And by Good A. (2018) Toward nitrogen-fixing plants. Science 359 (6378): 869-870, DOI: 10.1126/science.aas8737]. It has been compiled by the Royal Society of Biology (UK) as one of The Big Biology Breakthroughs of 2017. https://www.rsb.org.uk/about-us/mysociety/158-biologist/features/1881-big-biology-breakthroughs-2017
6. Dai, X., Zhu, M., Warren, M., Balakrishnan, R., Patsalo, V., Okano, H., Williamson, J.R., Fredrick, K., Wang, Y.P.* Hwa, T.* Reduction of translating ribosomes enables Escherichia coli to maintain elongation rates during slow growth. Nature Microbiology 2016, 2:16231. doi: 10.1038/nmicrobiol.2016.231
7. Zhu, M., Dai, X.,* and Wang, Y.P.* Real time determination of bacterial in vivo ribosome translation elongation speed based on LacZ complementation system. Nucleic Acids Res. 2016, 44 (20): e155. doi: 10.1093/nar/gkw698.
8. Tian, Z.X*., Yi, X.X., Cho, A., O`Gara, F., and Wang, Y.P.* CpxR activates MexAB-OprM efflux pump expression and enhances antibiotic resistance in both laboratory and clinical nalB-type isolates of Pseudomonas aeruginosa. PLOS Pathogens 2016, 12(10):e1005932. doi: 10.1371/journal.ppat.1005932.
9. Wang, J., Yan, D., Dixon, R.*, and Wang, Y.P.* Deciphering the principles of bacterial nitrogen dietary preferences: a strategy for nutrient containment. mBio 2016, 7(4):e00792-16. doi:10.1128/mBio.00792-16.
10. Yang, Y., Darbari, V.C, Zhang, N., Lu, D., Glyde, R., Wang, Y.P., Winkelman, J., Gourse, R.L., Murakami, K.S., Buck, M., Zhang, X.*, Structures of the RNA polymerase-σ54 reveal new and conserved regulatory strategies. Science, 2015, 349: 882-885 doi: 10.1126/science.aab1478